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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00065263-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00065263-M01, RRID:AB_2269156
- Product name
- Mouse Monoclonal PYCRL Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PYCRL (4F11)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Genomic alterations underlie a pan-cancer metabolic shift associated with tumour hypoxia.
PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.
Functional specialization in proline biosynthesis of melanoma.
Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
Genomic alterations underlie a pan-cancer metabolic shift associated with tumour hypoxia.
Haider S, McIntyre A, van Stiphout RG, Winchester LM, Wigfield S, Harris AL, Buffa FM
Genome biology 2016 Jun 29;17(1):140
Genome biology 2016 Jun 29;17(1):140
PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.
Zaki MS, Bhat G, Sultan T, Issa M, Jung HJ, Dikoglu E, Selim L, G Mahmoud I, Abdel-Hamid MS, Abdel-Salam G, Marin-Valencia I, Gleeson JG
Annals of neurology 2016 Jul;80(1):59-70
Annals of neurology 2016 Jul;80(1):59-70
Functional specialization in proline biosynthesis of melanoma.
De Ingeniis J, Ratnikov B, Richardson AD, Scott DA, Aza-Blanc P, De SK, Kazanov M, Pellecchia M, Ronai Z, Osterman AL, Smith JW
PloS one 2012;7(9):e45190
PloS one 2012;7(9):e45190
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL expression in transfected 293T cell line by PYCRL monoclonal antibody (M01), clone 4F11.Lane 1: PYCRL transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - PYCRL monoclonal antibody (M01), clone 4F11 Analysis of PYCRL expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PYCRL Antibody (4F11) [H00065263-M01] - Detection limit for recombinant GST tagged PYCRL is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of monoclonal antibody to PYCRL on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL transfected lysate using anti-PYCRL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PYCRL monoclonal antibody.
