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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- MA5-47060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DRD2 Recombinant Rabbit Monoclonal Antibody (HL1478)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1478
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DRD2 in unboiled mouse tissue extract (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with DRD2 Monoclonal antibody (Product # MA5-47060) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DRD2 in various tissue extracts (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with DRD2 Monoclonal antibody (Product # MA5-47060) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG secondary antibody. Competitors antibody (Ab5084P) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DRD2 in Human tissue extract (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with DRD2 Monoclonal antibody (Product # MA5-47060) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DRD2 in unboiled SH-SY5Y whole cell and membrane extracts (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with DRD2 Monoclonal antibody (Product # MA5-47060) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. WCE: whole cell extract; ME: membrane extract.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DRD2 in Whole cell extract (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with DRD2 Monoclonal antibody (Product # MA5-47060) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DRD2 in SH-SY5Y cells. Cells were fixed in ice-cold MeOH for 5 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47060) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI Scale bar= 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DRD2 in DIV9 rat E18 primary cortical neuron cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47060) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DRD2 in paraffin-embedded rat brain. Samples were incubated in DRD2 Monoclonal antibody (Product # MA5-47060). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DRD2 in paraffin-embedded rat brain. Samples were incubated in DRD2 Monoclonal antibody (Product # MA5-47060). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DRD2 in paraffin-embedded mouse brain. Samples were incubated in DRD2 Monoclonal antibody (Product # MA5-47060). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
