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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- EB06268 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB06268, RRID:AB_2215211
- Product name
- Goat anti-VPS35 / MEM3 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-VPS35 / MEM3 Antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
C-SPESEGPIYEGLIL- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06268 staining (0.05µg/ml) of Human Brain Cerebellum lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB06268 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3µg/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining.
