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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA302699 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA302699, RRID:AB_2215214
- Product name
- Goat Polyclonal Antibody against VPS35 / MEM3
- Antibody type
- Polyclonal
- Description
- Goat Polyclonal Antibody against VPS35 / MEM3
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- VPS35
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302699 staining (0.05ug/ml) of Human Brain Cerebellum lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302699 staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302699 staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302699 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3ug/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining.
- Validation comment
- IHC
