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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- AP16208PU-T - Provider product page
- Provider
- OriGene
- Product name
- Goat Polyclonal Anti-VPS35 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Goat Polyclonal Anti-VPS35 Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 20ug
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- staining (0.05 ug/ml) of Human Brain Cerebellum lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- staining (0.05 ug/ml)?of?PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- staining (0.05 ug/ml)?of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A) Tris/EDTA buffer pH9 at 3ug/ml or B) Citrate buffer pH6 at 10 ug/ml. HRP-staining.
- Validation comment
- IHC
