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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB7499 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7499, RRID:AB_1581876
- Product name
- VPS35 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of VPS35.
- Antigen sequence
C-SPESEGPIYEGLIL- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer.
Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA.
EHD1 interacts with retromer to stabilize SNX1 tubules and facilitate endosome-to-Golgi retrieval.
Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L
Nature 2009 Jun 25;459(7250):1085-90
Nature 2009 Jun 25;459(7250):1085-90
Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA.
Nielsen MS, Gustafsen C, Madsen P, Nyengaard JR, Hermey G, Bakke O, Mari M, Schu P, Pohlmann R, Dennes A, Petersen CM
Molecular and cellular biology 2007 Oct;27(19):6842-51
Molecular and cellular biology 2007 Oct;27(19):6842-51
EHD1 interacts with retromer to stabilize SNX1 tubules and facilitate endosome-to-Golgi retrieval.
Gokool S, Tattersall D, Seaman MN
Traffic (Copenhagen, Denmark) 2007 Dec;8(12):1873-86
Traffic (Copenhagen, Denmark) 2007 Dec;8(12):1873-86
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VPS35 polyclonal antibody (Cat # PAB7499) staining (1 ug/mL) of human cerebellum lysate (RIPA buffer, 35 ug total protein per lane) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VPS35 polyclonal antibody (Cat # PAB7499) staining of paraffin embedded human testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A) Tris/EDTA buffer pH9 at 3 ug/mL or B) Citrate buffer pH6 at 10ug/mL ., HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
