AF6278

antibody from R&D Systems

Targeting: PGPEP1 Pcp, PGP, PGP-I, PGPI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6278

Provider

R&D Systems

Product name

Human/Mouse PGPEP-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PGPEP-1 in direct ELISAs and, human and mouse PGPEP-1 in Western blots.

Reactivity

Human, Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9NXJ5


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse PGPEP-1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PGPEP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6278) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PGPEP-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.