H00003340-M01

antibody from Abnova Corporation

Targeting: NDST1 HSST, NST1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003340-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003340-M01, RRID:AB_606644

Product name

NDST1 monoclonal antibody (M01), clone 1G10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant NDST1.

Antigen sequence

LYGWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKP
VQAATPSRTDPLVLVFVESLYSQLGQEVVAILESS
RFKYRTEIAPGKGDMPTLTDKGRGRFALI


Isotype

IgG

Antibody clone number

1G10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Epac increases melanoma cell migration by a heparan sulfate-related mechanism.

Baljinnyam E, Iwatsubo K, Kurotani R, Wang X, Ulucan C, Iwatsubo M, Lagunoff D, Ishikawa Y
American journal of physiology. Cell physiology 2009 Oct;297(4):C802-13

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NDST1 monoclonal antibody (M01), clone 1G10 Western Blot analysis of NDST1 expression in A-549 ( Cat # L025V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NDST1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to NDST1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol