Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A01868-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-GAP43 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for GAP43 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Low-Dose LPS Modulates Microglia/Macrophages Phenotypic Transformation to Amplify Rehabilitation Effects in Chronic Spinal Cord Injured (CSCI) Mice.
Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury.
Adsorptive properties of graphene oxide on vitamin B12 and their effect on the promotion of peripheral nerve regeneration.
Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated Protein-43 in Rats.
Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system.
Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo.
Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways.
Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration.
β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice.
Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection.
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats.
Zhong J, He Y, Zhao Q, Luo H, Zhang Q, Tian Y, Liu Y, Yang C, Yin Y, Yu L, Pan L, Tan B
Molecular neurobiology 2024 Sep;61(9):6484-6500
Molecular neurobiology 2024 Sep;61(9):6484-6500
Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury.
Wang Y, Su H, Zhong J, Zhan Z, Zhao Q, Liu Y, Li S, Wang H, Yang C, Yu L, Tan B, Yin Y
Molecular biomedicine 2023 Nov 28;4(1):44
Molecular biomedicine 2023 Nov 28;4(1):44
Adsorptive properties of graphene oxide on vitamin B12 and their effect on the promotion of peripheral nerve regeneration.
Niu QF, Wang QL, Tong ZX, Tong L, Tong XJ
Neurological research 2019 Mar;41(3):282-288
Neurological research 2019 Mar;41(3):282-288
Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated Protein-43 in Rats.
Liu Z, Liu H, Zeng ZH
BioMed research international 2018;2018:8697913
BioMed research international 2018;2018:8697913
Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system.
Yuan Q, Sun L, Yu H, An C
Bioscience, biotechnology, and biochemistry 2017 Jul;81(7):1335-1342
Bioscience, biotechnology, and biochemistry 2017 Jul;81(7):1335-1342
Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo.
Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J
Scientific reports 2017 Jan 5;7:39869
Scientific reports 2017 Jan 5;7:39869
Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways.
Tao T, Feng JZ, Xu GH, Fu J, Li XG, Qin XY
Cellular and molecular neurobiology 2017 Apr;37(3):417-426
Cellular and molecular neurobiology 2017 Apr;37(3):417-426
Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration.
Dingyu W, Fanjie M, Zhengzheng D, Baosheng H, Chao Y, Yi P, Huiwen W, Jun G, Gang H
Molecular neurobiology 2016 Sep;53(7):4582-95
Molecular neurobiology 2016 Sep;53(7):4582-95
β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice.
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu DH, Deng W, Lai Y, Zhou JH, Zhang SX, Lin WW, Chen DF
Brain research 2014 Mar 13;1552:41-54
Brain research 2014 Mar 13;1552:41-54
Cograft of neural stem cells and schwann cells overexpressing TrkC and neurotrophin-3 respectively after rat spinal cord transection.
Wang JM, Zeng YS, Wu JL, Li Y, Teng YD
Biomaterials 2011 Oct;32(30):7454-68
Biomaterials 2011 Oct;32(30):7454-68
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats.
Liu J, He QJ, Zou W, Wang HX, Bao YM, Liu YX, An LJ
Brain research 2006 Dec 6;1123(1):68-79
Brain research 2006 Dec 6;1123(1):68-79
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of GAP43 using anti-GAP43 antibody (A01868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (Catalog # A01868-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 45KD. The expected band size for GAP43 is at 24KD.
- Additional image