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- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 16757-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16757-1-AP, RRID:AB_2112098
- Product name
- GPT2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GPT2 antibody (Cat. #16757-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.
Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.
Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition.
T Cell Activation Depends on Extracellular Alanine.
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.
Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.
Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.
Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.
Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, Niu Y, Xu X, Guo S, Yang Y, Wang Z, Xue X, Gu C, Fang W, Sun J, Yu Y, Wang J
Theranostics 2021;11(12):5650-5674
Theranostics 2021;11(12):5650-5674
Enhanced glutamine utilization mediated by SLC1A5 and GPT2 is an essential metabolic feature of colorectal signet ring cell carcinoma with therapeutic potential.
Wang R, Xiang W, Xu Y, Han L, Li Q, Dai W, Cai G
Annals of translational medicine 2020 Mar;8(6):302
Annals of translational medicine 2020 Mar;8(6):302
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity protein phosphatase 1.
Cai X, Qu L, Yang J, Xu J, Sun L, Wei X, Qu X, Bai T, Guo Z, Zhu Y
Cancer medicine 2020 Aug;9(16):6009-6019
Cancer medicine 2020 Aug;9(16):6009-6019
Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition.
Boku S, Watanabe M, Sukeno M, Yaoi T, Hirota K, Iizuka-Ohashi M, Itoh K, Sakai T
Cancers 2020 Apr 30;12(5)
Cancers 2020 Apr 30;12(5)
T Cell Activation Depends on Extracellular Alanine.
Ron-Harel N, Ghergurovich JM, Notarangelo G, LaFleur MW, Tsubosaka Y, Sharpe AH, Rabinowitz JD, Haigis MC
Cell reports 2019 Sep 17;28(12):3011-3021.e4
Cell reports 2019 Sep 17;28(12):3011-3021.e4
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
Mitochondrial GPT2 plays a pivotal role in metabolic adaptation to the perturbation of mitochondrial glutamine metabolism.
Kim M, Gwak J, Hwang S, Yang S, Jeong SM
Oncogene 2019 Jun;38(24):4729-4738
Oncogene 2019 Jun;38(24):4729-4738
Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.
Hong R, Zhang W, Xia X, Zhang K, Wang Y, Wu M, Fan J, Li J, Xia W, Xu F, Chen J, Wang S, Zhan Q
Molecular oncology 2019 Apr;13(4):959-977
Molecular oncology 2019 Apr;13(4):959-977
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Chen Q, Kirk K, Shurubor YI, Zhao D, Arreguin AJ, Shahi I, Valsecchi F, Primiano G, Calder EL, Carelli V, Denton TT, Beal MF, Gross SS, Manfredi G, D'Aurelio M
Cell metabolism 2018 May 1;27(5):1007-1025.e5
Cell metabolism 2018 May 1;27(5):1007-1025.e5
Metabolic consequences of HIF silencing in a triple negative human breast cancer xenograft.
Bharti SK, Mironchik Y, Wildes F, Penet MF, Goggins E, Krishnamachary B, Bhujwalla ZM
Oncotarget 2018 Mar 16;9(20):15326-15339
Oncotarget 2018 Mar 16;9(20):15326-15339
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545
Nature communications 2018 Feb 7;9(1):545
Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Cai WF, Zhang C, Wu YQ, Zhuang G, Ye Z, Zhang CS, Lin SC
Cell research 2018 Aug;28(8):865-867
Cell research 2018 Aug;28(8):865-867
Glutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.
Cao Y, Lin SH, Wang Y, Chin YE, Kang L, Mi J
Theranostics 2017;7(12):3021-3033
Theranostics 2017;7(12):3021-3033
Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.
Ouyang Q, Nakayama T, Baytas O, Davidson SM, Yang C, Schmidt M, Lizarraga SB, Mishra S, Ei-Quessny M, Niaz S, Gul Butt M, Imran Murtaza S, Javed A, Chaudhry HR, Vaughan DJ, Hill RS, Partlow JN, Yoo SY, Lam AT, Nasir R, Al-Saffar M, Barkovich AJ, Schwede M, Nagpal S, Rajab A, DeBerardinis RJ, Housman DE, Mochida GH, Morrow EM
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 20;113(38):E5598-607
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 20;113(38):E5598-607
Transcriptomic and metabolic analyses reveal salvage pathways in creatine-deficient AGAT(-/-) mice.
Stockebrand M, Nejad AS, Neu A, Kharbanda KK, Sauter K, Schillemeit S, Isbrandt D, Choe CU
Amino acids 2016 Aug;48(8):2025-39
Amino acids 2016 Aug;48(8):2025-39
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP( GPT2 Antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 16757-1-AP(GPT2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GPT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GPT2. This antibody recognizes human,mouse,rat antigen. The GPT2 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
