15557-1-AP

antibody from Invitrogen Antibodies

Targeting: DIRAS2 Di-Ras2, DKFZp761C07121

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15557-1-AP

Provider

Invitrogen Antibodies

Product name

DIRAS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPEQSNDYR VAVFGAGGVG KSSLVLRFVK GTFRESYIPT VEDTYRQVIS CDKSICTLQI TDTTGSHQFP AMQRLSISKG HAFILVYSIT SRQSLEELKP IYEQICEIKG DVESIPIMLV GNKCDESPSR EVQSSEAEAL ARTWKCAFME TSAKLNHNVK ELFQELLNLE KRRTVSLQID GKKSKQQKRK EKLKGKCVIM (1-199 aa encoded by BC008065)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15557-1-AP (DIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using DIRAS2 antibody 15557-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.05ug DIRAS2 antibody (15557-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IGG with dilution 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DIRAS2 (IP:15557-1-AP, 4ug; Detection:15557-1-AP 1:1000) with mouse brain tissue lysate 4400ug.