LS-C744795

antibody from LSBio

Targeting: PGA3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C744795

Provider

LSBio

Product name

PGA3 Antibody LS-C744795

Antibody type

Polyclonal

Reactivity

Porcine

Host

Goat

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot of Goat anti-Pepsin Antibody. Lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Pepsin primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 35 kDa, 35 kDa for Pepsin. Other band(s): None.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of goat anti-pepsin antibody Antigen Retrieval: HIER pH6.2 10X Primary Antibody: Goat Anti-Pepsin 1 hr 1:500 Secondary Antibody: Swine anti-goat HRP 45 min followed by Staining with DAB. Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).