GTX54945

antibody from GeneTex

Targeting: ADCY5 AC5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX54945

Provider

GeneTex

Product name

Adenylate cyclase 5/6 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of Adenylate Cyclase 5/6 expression in HeLa (A); Raw264.7 (B); PC12 (C); rat brain (D) whole cell lysates.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Adenylate Cyclase 5/6 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjμgated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).