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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- A305-792A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM214 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TMEM214 by western blot. Samples: Whole cell lysate (15 µg) from Hep-G2, A-549, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TMEM214 antibody A305-792A-M (lot A305-792A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TMEM214 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TMEM214 antibody (Product # A305-792A-T; lot A305-792A-T-1) used for IP at 100 µL per reaction. TMEM214 was also immunoprecipitated by rabbit anti-TMEM214 antibody (Product # A305-793A-T). For blotting immunoprecipitated TMEM214, Product # A305-792A-T was used at 1:100. Detection: Chemiluminescence with an exposure time of 1 second.
