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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09154PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-FKBP8 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-FKBP8 Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 2.3 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Western blot using protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4¡ã C, overnight.
- Validation comment
- WB
