PAB10013

antibody from Abnova Corporation

Targeting: FKBP8 FKBP38, FKBPr38

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10013

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10013, RRID:AB_1674769

Product name

FKBP8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of FKBP8.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Hepatitis C virus RNA replication is regulated by FKBP8 and Hsp90.

Okamoto T, Nishimura Y, Ichimura T, Suzuki K, Miyamura T, Suzuki T, Moriishi K, Matsuura Y
The EMBO journal 2006 Oct 18;25(20):5015-25

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Bcl-2 localized at the nuclear compartment induces apoptosis after transient overexpression.

Portier BP, Taglialatela G
The Journal of biological chemistry 2006 Dec 29;281(52):40493-502

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Hepatitis C virus non-structural protein NS5A interacts with FKBP38 and inhibits apoptosis in Huh7 hepatoma cells.

Wang J, Tong W, Zhang X, Chen L, Yi Z, Pan T, Hu Y, Xiang L, Yuan Z
FEBS letters 2006 Aug 7;580(18):4392-400

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using FKBP8 polyclonal antibody (Cat # PAB10013) shows detection of exogenous FKBP8 in 50 ug of HEK293T whole cell lysate (Lane 1).The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane.The membrane was probed with the primary antibody at a 1 : 1,000 dilution in 5% BLOTTO at 4°C, overnight.Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.