PA5-96573

antibody from Invitrogen Antibodies

Targeting: FKBP8 FKBP38, FKBPr38

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96573

Provider

Invitrogen Antibodies

Product name

FKBP8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: QPPAEEAEQP GALAREFLAA MEPEPAPAPA PEEWLDILGN GLLRKKTLVP GPPGSSRPVK GQVVTVHLQT SLENGTRVQE EPELVFTLGD CDVIQALDLS VPLMDVGETA MVTADSKYCY GPQGSRSPYI PPHAALCLEV TLKTAVDGPD LEMLTGQERV ALANRKRECG NAHYQRADFV LAANSYDLAI KAITSSAKVD MTFEEEAQLL Q; Positive Samples: Jurkat, THP-1, BT-474, HeLa, HL-60, Mouse liver, Mouse brain, Mouse eye, Rat brain; Cellular Location: Cytoplasmic side, Mitochondrion membrane, Single-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.35 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FKBP8 in extracts of various cell lines. Samples were incubated with FKBP8 Polyclonal antibody (Product # PA5-96573) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of FKBP8 was performed in A549 cells using FKBP8 Polyclonal Antibody (Product # PA5-96573).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of FKBP8 in A549 cells. Samples were incubated with FKBP8 Polyclonal antibody (Product # PA5-96573). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of FKBP8 in 200 μg extracts of HeLa cells. Samples were precipitated with 1 μg FKBP8 Polyclonal antibody (Product # PA5-96573). Western blot was performed from the immunoprecipitate using FKBP8 Polyclonal antibody (Product # PA5-96573) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of FKBP8 was performed in 200 µg extracts of HeLa cells using FKBP8 Polyclonal Antibody (Product # PA5-96573). Western blot was performed from the immunoprecipitate using FKBP8 Polyclonal Antibody at a dilution of 1:1000.