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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09154PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09154PU-S, RRID:AB_2035481
- Product name
- anti FKBP8 / FKBP38
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region of human FKBP8 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 2.3 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4° C, overnight.
