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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MAB4960 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Angiopoietin-like Protein 7/ANGPTL7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-like 1, 5, 7, recombinant mouse (rm) Angiopoietin-like 2, 3, 4, 6, rhAngiopoietin-1, rhAngiopoietin-4, rmAngiopoietin-2, or rmAngiopoietin-3 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8R1Q3- Isotype
- IgG
- Antibody clone number
- 538401
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Angiopoietin-like Protein 7/ANGPTL7 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Angiopoietin-like Protein 7/ANGPTL7 Monoclonal Antibody (Catalog # MAB4960) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 7/ANGPTL7 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-like Protein 7/ANGPTL7 in Mouse Brain. Angiopoietin-like Protein 7/ANGPTL7 was detected in perfusion fixed frozen sections of mouse brain using Rat Anti-Mouse Angiopoietin-like Protein 7/ANGPTL7 Monoclonal Antibody (Catalog # MAB4960) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-like Protein 7/ANGPTL7 in Mouse Brain. Angiopoietin-like Protein 7/ANGPTL7 was detected in perfusion fixed frozen sections of mouse brain using Rat Anti-Mouse Angiopoietin-like Protein 7/ANGPTL7 Monoclonal Antibody (Catalog # MAB4960) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
