H00022827-M20

antibody from Novus Biologicals

Targeting: PUF60 FIR, RoBPI, SIAHBP1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00022827-M20

Provider

Novus Biologicals

Proper citation

Novus Cat#H00022827-M20, RRID:AB_2253216

Product name

Mouse Monoclonal PUF60 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Reacts with poly-U binding splicing factor 60KDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: PUF60 Antibody (1C1) [H00022827-M20] - Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of monoclonal antibody to PUF60 on HeLa cell. Antibody concentration 10 ug/ml