Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-27821 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PUF60 Monoclonal Antibody (GT3510)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT3510
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PUF60 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with PUF60 monoclonal antibody (Product # MA5-27821) using a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PUF60 in rat brain lysate using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with PUF60 monoclonal antibody (Product # MA5-27821) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Monoclonal Antibody (GT3510) detects PUF60 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. PUF60 Monoclonal Antibody (GT3510) (Product # MA5-27821) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Monoclonal Antibody (GT3510) detects PUF60 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract.7.5 % SDS-PAGE. PUF60 Monoclonal Antibody (GT3510) (Product # MA5-27821) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Monoclonal Antibody (GT3510) detects PUF60 protein by Western blot analysis. A. 50 µg rat brain lysate/extract.7.5 % SDS-PAGE. PUF60 Monoclonal Antibody (GT3510) (Product # MA5-27821) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Monoclonal Antibody (GT3510) detects PUF60 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 Monoclonal Antibody (GT3510) (Product # MA5-27821) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Monoclonal Antibody (GT3510) detects PUF60 protein at on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. PUF60 Monoclonal Antibody (GT3510) (Product # MA5-27821) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Monoclonal Antibody (GT3510) detects PUF60 protein at on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. PUF60 Monoclonal Antibody (GT3510) (Product # MA5-27821) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.