NB300-263

antibody from Novus Biologicals

Targeting: UBA52 CEP52, HUBCEP52, L40, MGC126879, MGC126881, MGC57125, RPL40

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-263

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-263, RRID:AB_10002171

Product name

Rabbit Polyclonal UBA52 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Bovine

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

The ubiquitin ligase itch regulates apoptosis by targeting thioredoxin-interacting protein for ubiquitin-dependent degradation.

Zhang P, Wang C, Gao K, Wang D, Mao J, An J, Xu C, Wu D, Yu H, Liu JO, Yu L
The Journal of biological chemistry 2010 Mar 19;285(12):8869-79

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UBA52 Antibody [NB300-263] - Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified human ubiquitin was applied in decreasing amounts from 5 to 0.1 mcg. Antibody: Affinity purified rabbit anti-Ubiquitin antibody NB 300-263 used at 0.4 mcg/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: UBA52 Antibody [NB300-263] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Ubiquitin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB