PA5-65039

antibody from Invitrogen Antibodies

Targeting: NRBP2 DKFZp434P086

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-65039

Provider

Invitrogen Antibodies

Product name

NRBP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MELDKFLEDVR NGIYPLMNFA ATRPLGLPRV LAPPPEEVQK AKTPTPEPFD SETRKVIQMQ CNLERSEDKA RWHLTLLLVL EDRLHRQLTY DLLPTDSAQD LASELVHYGF LHEDDRMKLA AFLESTF

Concentration

0.1 mg/mL

References

Submitted references

GATA binding protein 1 recruits histone deacetylase 2 to the promoter region of nuclear receptor binding protein 2 to affect the tumor microenvironment and malignancy of thyroid carcinoma.

Li M, Jiang H, Chen S, Ma Y
Bioengineered 2022 Apr;13(4):11320-11341

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NRBP2 in human cell line RT-4 and human cell line U-251 MG. Samples were probed using a NRBP2 Polyclonal Antibody (Product # PA5-65039).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NRBP2 in human cell line U-2 OS shows localization to cytosol. Samples were probed using a NRBP2 Polyclonal Antibody (Product # PA5-65039).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Overexpression of NRBP2 reduces activity of TC cells in vitro . A-B, mRNA (a) and protein (b) levels of NRBP2 in TPC-1 and CAL62 cells after oe-NRBP2 transfection examined by RT-qPCR and western blot assays; (c), DNA replication ability of TPC-1 and CAL62 cells examined by the EdU labeling assay; (d), colony formation ability of TPC-1 and CAL62 cells determined by the colony formation assay; (e), apoptosis rate in TPC-1 and CAL62 cells determined by flow cytometry. Three repetitions were performed. ** p < 0.01.