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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-78012 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2 Predicted Reactivity: Mouse (92%), Xenopus laevis (80%), Bovine (94%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IARS in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with IARS polyclonal antibody (Product # PA5-78012) using a dilution of 1:4000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IARS Polyclonal Antibody detects IARS protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with IARS Polyclonal Antibody (Product # PA5-78012) diluted at 1:4,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IARS Polyclonal Antibody detects IARS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IARS Polyclonal Antibody (Product # PA5-78012) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IARS protein from 293T whole cell extracts using 5 µg of IARS Polyclonal Antibody (Product # PA5-78012). Western blot analysis was performed using IARS Polyclonal Antibody (Product # PA5-78012) diluted at 1:2500.
