AF570

antibody from R&D Systems

Targeting: COL18A1 KNO, KNO1, KS

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF570

Provider

R&D Systems

Product name

Mouse Endostatin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Endostatin in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endostatin is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAA19787


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.

Öhlund D, Franklin O, Lundberg E, Lundin C, Sund M
BMC cancer 2013 Mar 26;13:154

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Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.

Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
British journal of cancer 2011 Nov 8;105(10):1615-24

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Cationic liposome coupled endostatin gene for treatment of peritoneal colon cancer.

Lan KL, Ou-Yang F, Yen SH, Shih HL, Lan KH
Clinical & experimental metastasis 2010 May;27(5):307-18

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Endostatin peptide, an inhibitor of angiogenesis, prevents the progression of peritoneal sclerosis in a mouse experimental model.

Tanabe K, Maeshima Y, Ichinose K, Kitayama H, Takazawa Y, Hirokoshi K, Kinomura M, Sugiyama H, Makino H
Kidney international 2007 Feb;71(3):227-38

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Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.

Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
The American journal of pathology 2006 Nov;169(5):1767-83

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Endostatin in Mouse Intestine. Endostatin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF570) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lacteals. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.