- SP5230P - Acris Antibodies GmbH COL18A1 antibody

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Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of A431 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Cat.-No SP5230P) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ÂºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of 293T cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Cat.-NoÂ SP5230P) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ÂºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Cat.-No SP5230P) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ÂºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunohistochemistry analysis of Endostatin showing positive staining in the secretion of paraffin-treated Human kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an endostatin polyclonal antibody (Cat.-No SP5230P) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4Â°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

SP5230P