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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- 21890002-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal DCLK3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product is specific for Human DCAMKL3.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DCLK3 Antibody [21890002] - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa(138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa(138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DCLK3 Antibody [21890002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DCLK3 Antibody [21890002]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DCLK3 Antibody [21890002] - Normal tissues generally displayed weak to moderate cytoplasmic and occasional nuclear positivity. Ciliated cells in airways and fallopian tube exhibited distinct membranous staining. Cells for example in liver, prostate and spleen were negative. Colo-rectal and endometrial cancers exhibited some moderately stained cases while malignant gliomas, ovarian and stomach cancers showed several weakly immunoreactive samples. Remaining malignant tissues were mainly negative. Image and statement courtesy of the Human Protein Atlas (HPA).
