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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF2984a - Provider product page
- Provider
- Abgent
- Product name
- "EPM2AIP1 Antibody (internal region, near C-Term)"
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide-affinity purified goat antibody
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -705°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human EPM2AIP1 with DYKDDDDK tag probed with AF2984a (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AF2984a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "AF2984a (5 µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining."
