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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00009265-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009265-M01, RRID:AB_547383
- Product name
- Mouse Monoclonal Cytohesin 3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PSCD3 - pleckstrin homology, Sec7 and coiled-coil domains 3 Please note this antibody was made to Human CYTH3 accession number Q96HS5. This is the 179 amino acid uncharacterized human CYTH3 protein that shares 99% homology with the C-terminal region (221-400) of isoform 1 and 100% homology with the C-terminal region (221-399) of isoform 2 of the characterized human CYTH3 protein (accession number O43739).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - Analysis of PSCD3 expression in transfected 293T cell line by PSCD3 monoclonal antibody (M01), clone 6D3-1A9.Lane 1: PSCD3 transfected lysate(46.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - PSCD3 monoclonal antibody (M01), clone 6D3-1A9 Analysis of PSCD3 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - Detection limit for recombinant GST tagged PSCD3 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Cytohesin 3 Antibody (6D3-1A9) [H00009265-M01] - Analysis of monoclonal antibody to PSCD3 on HeLa cell. Antibody concentration 10 ug/ml.
