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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-58810 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SVPGSKPTFE LSADEVELGL GIHGEAGVRR IKMATADEIV KLMLDHMTNT TNASHVPVQP GSSVVMMVNN LGGLSFLELG IIADATVRSL EGRGVKIARA
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAK in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a DAK Polyclonal Antibody (Product # PA5-58810).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DAK in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a DAK Polyclonal Antibody (Product # PA5-58810).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAK in human kidney using DAK Polyclonal Antibody (Product # PA5-58810).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAK in human liver using DAK Polyclonal Antibody (Product # PA5-58810).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAK in human duodenum and skeletal muscle tissues using DAK Polyclonal Antibody (Product # PA5-58810). Corresponding TKFC RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAK in human duodenum, kidney, liver and skeletal muscle using DAK Polyclonal Antibody (Product # PA5-58810) (A) shows similar protein distribution across tissues to an independent DAK Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAK in human duodenum using DAK Polyclonal Antibody (Product # PA5-58810) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAK in human skeletal muscle using DAK Polyclonal Antibody (Product # PA5-58810) shows low expression as expected.
