H00050810-M02

antibody from Abnova Corporation

Targeting: HDGFL3 Hdgfrp3, HRP-3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00050810-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00050810-M02, RRID:AB_10553745

Product name

HDGFRP3 monoclonal antibody (M02), clone 1D1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HDGFRP3.

Antigen sequence

MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEG
AVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKD
KFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQ
SSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNE
KAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEE
NKSSSEGGDAGNDTRNTTSDLQKTSEGT


Isotype

IgG

Antibody clone number

1D1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HDGFRP3 expression in transfected 293T cell line by HDGFRP3 monoclonal antibody (M02), clone 1D1.Lane 1: HDGFRP3 transfected lysate(22.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HDGFRP3 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to HDGFRP3 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol