PA5-117954

antibody from Invitrogen Antibodies

Targeting: FNIP2 FNIPL, KIAA1450, MAPO1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117954

Provider

Invitrogen Antibodies

Product name

FNIP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FNIP2 Polyclonal Antibody (Product # PA5-117954) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: U-251MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FNIP2 Polyclonal Antibody (Product # PA5-117954) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: U-251MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human FNIP2 in human kidney with FNIP2 Polyclonal Antibody (Product # PA5-117954, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FNIP2 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with FNIP2 Polyclonal Antibody (Product # PA5-117954) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: FNIP2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 95 kDa. Observed band size: 95 kDa.