PAB28707

antibody from Abnova Corporation

Targeting: MED15 Arc105, CAG7A, PCQAP, TIG-1, TNRC7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28707

Provider

Abnova Corporation

Product name

MED15 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MED15.

Antigen sequence

QKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKT
RDEYLSLVARLIIHFRDIHNKKSQASVSDPMNALQ
SLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQ
PMSLSGQP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4Lane 2: Human cell line U-251MG spLane 3: Human cell line A-431with MED15 polyclonal antibody (Cat # PAB28707) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with MED15 polyclonal antibody (Cat # PAB28707) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with MED15 polyclonal antibody (Cat # PAB28707) shows strong nuclear and cytoplasmic positivity in trophoblastic cells at 1:10-1:20 dilution.

Validation comment

Immunohistochemistry