H00051586-M02

antibody from Abnova Corporation

Targeting: MED15 Arc105, CAG7A, PCQAP, TIG-1, TNRC7

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051586-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051586-M02, RRID:AB_530166

Product name

PCQAP monoclonal antibody (M02), clone 4A4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PCQAP.

Antigen sequence

MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHS
KSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIH
NKKSQASVSDPMNALQSL


Isotype

IgG

Antibody clone number

4A4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.

Ding N, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Boyer TG
The Journal of biological chemistry 2009 Jan 30;284(5):2648-2656

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TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.

Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
Nature cell biology 2008 Jul;10(7):837-48

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MED15 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PCQAP on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol