26072-1-AP

antibody from Invitrogen Antibodies

Targeting: PARP10 ARTD10, FLJ14464

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

26072-1-AP

Provider

Invitrogen Antibodies

Product name

PARP10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QEEAAALRQ ALTLSLLEQP PLEAEEPPDG GTDGKAQLVV HSAFEQDVEE LDRALRAALE VHVQEETVGP WRRTLPAELR ARLERCHGVS VALRGDCTIL RGFGAHPARA ARHLVALLAG PWDQSLAFPL AASGPTLAGQ TLKGPWNNLE RLAENTGEFQ EVVRAFYDTL DAARSSIRVV RVERVSHPLL QQQYELYRER LLQRCERRPV EQVLYHGTTA PAVPDICAHG FNRSFCGRNA TVYGKGVYFA RRASLSVQDR YSPPNADGHK AVFVARVLTG DYGQGRRGLR APPLRGPGHV LLRYDSAVDC ICQPSIFVIF HDTQALPTHL ITCEHVPRAS PDDPSGLPGR SPDT (673-1025 aa encoded by BC136542)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.39 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP ( PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 26072-1-AP (PARP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 26072-1-AP (PARP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.