H00009693-M01

antibody from Novus Biologicals

Targeting: RAPGEF2 DKFZP586O1422, KIAA0313, PDZ-GEF1, PDZGEF1, RA-GEF

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

H00009693-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00009693-M01, RRID:AB_2177456

Product name

Mouse Monoclonal RAPGEF2 Antibody

Antibody type

Monoclonal

Description

IgG purified. RAPGEF2 - Rap guanine nucleotide exchange factor (GEF) 2

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Rap2A links intestinal cell polarity to brush border formation.

Gloerich M, ten Klooster JP, Vliem MJ, Koorman T, Zwartkruis FJ, Clevers H, Bos JL
Nature cell biology 2012 Aug;14(8):793-801

---

Epac1 and PDZ-GEF cooperate in Rap1 mediated endothelial junction control.

Pannekoek WJ, van Dijk JJ, Chan OY, Huveneers S, Linnemann JR, Spanjaard E, Brouwer PM, van der Meer AJ, Zwartkruis FJ, Rehmann H, de Rooij J, Bos JL
Cellular signalling 2011 Dec;23(12):2056-64

---

PKC-theta selectively controls the adhesion-stimulating molecule Rap1.

Letschka T, Kollmann V, Pfeifhofer-Obermair C, Lutz-Nicoladoni C, Obermair GJ, Fresser F, Leitges M, Hermann-Kleiter N, Kaminski S, Baier G
Blood 2008 Dec 1;112(12):4617-27

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: RAPGEF2 Antibody (1E8) [H00009693-M01] - Detection limit for recombinant GST tagged RAPGEF2 is approximately 0.1ng/ml as a capture antibody.