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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-120840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 0 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: HepG2, U-251MG, Mouse skeletal muscle, Mouse heart, Mouse liver, Mouse testis, Mouse eye, Rat eye. The target is usually found in the following locations: Cell junction, Cell membrane, Multi-pass membrane protein, gap junction.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.83 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Aquaporin 0 in various cell lines using a Aquaporin 0 Polyclonal antibody (Product # PA5-120840) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Aquaporin 0 in human stomach using a Polyclonal antibody (Product # PA5-120840) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Aquaporin 0 in human stomach using a Polyclonal antibody (Product # PA5-120840) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Aquaporin 0 in human stomach using a Polyclonal antibody (Product # PA5-120840) at a dilution of 1:100.
