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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [24]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-24733 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Endophilin B1/Bif-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Neuronal susceptibility to beta-amyloid toxicity and ischemic injury involves histone deacetylase-2 regulation of endophilin-B1.
Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.
USP1 (ubiquitin specific peptidase 1) targets ULK1 and regulates its cellular compartmentalization and autophagy.
Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin.
Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.
Bif-1 promotes tumor cell migration and metastasis via Cdc42 expression and activity.
Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.
Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo.
Endophilin B2 promotes inner mitochondrial membrane degradation by forming heterodimers with Endophilin B1 during mitophagy.
The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs.
IFNg-induced Irgm1 promotes tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy.
Loss of endophilin-B1 exacerbates Alzheimer's disease pathology.
Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons.
Bif-1 suppresses breast cancer cell migration by promoting EGFR endocytic degradation.
Scratching beneath the surface: new insights into the functional properties of the lateral occipital area and parahippocampal place area.
Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy.
Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy.
GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.
Endophilin B1/Bif-1 stimulates BAX activation independently from its capacity to produce large scale membrane morphological rearrangements.
SRC directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis.
Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis.
Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
Endophilin B1 is required for the maintenance of mitochondrial morphology.
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Wang DB, Kinoshita C, Kinoshita Y, Sopher BL, Uo T, Lee RJ, Kim JK, Murphy SP, Dirk Keene C, Garden GA, Morrison RS
Brain pathology (Zurich, Switzerland) 2019 Mar;29(2):164-175
Brain pathology (Zurich, Switzerland) 2019 Mar;29(2):164-175
Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.
Cho SG, Xiao X, Wang S, Gao H, Rafikov R, Black S, Huang S, Ding HF, Yoon Y, Kirken RA, Yin XM, Wang HG, Dong Z
Journal of the American Society of Nephrology : JASN 2019 Jul;30(7):1174-1191
Journal of the American Society of Nephrology : JASN 2019 Jul;30(7):1174-1191
USP1 (ubiquitin specific peptidase 1) targets ULK1 and regulates its cellular compartmentalization and autophagy.
Raimondi M, Cesselli D, Di Loreto C, La Marra F, Schneider C, Demarchi F
Autophagy 2019 Apr;15(4):613-630
Autophagy 2019 Apr;15(4):613-630
Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin.
Marcassa E, Raimondi M, Anwar T, Eskelinen EL, Myers MP, Triolo G, Schneider C, Demarchi F
Biology open 2017 May 15;6(5):551-562
Biology open 2017 May 15;6(5):551-562
Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.
Serfass JM, Takahashi Y, Zhou Z, Kawasawa YI, Liu Y, Tsotakos N, Young MM, Tang Z, Yang L, Atkinson JM, Chroneos ZC, Wang HG
The Journal of biological chemistry 2017 Jun 16;292(24):10097-10111
The Journal of biological chemistry 2017 Jun 16;292(24):10097-10111
Bif-1 promotes tumor cell migration and metastasis via Cdc42 expression and activity.
Zhang C, Liu F, Chen H, Li N, Luo Z, Guo W, Huang D, Tang S, Wang H, Cheng S, Li Z, Wang H
Clinical & experimental metastasis 2017 Jan;34(1):11-23
Clinical & experimental metastasis 2017 Jan;34(1):11-23
Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.
Xu S, Cherok E, Das S, Li S, Roelofs BA, Ge SX, Polster BM, Boyman L, Lederer WJ, Wang C, Karbowski M
Molecular biology of the cell 2016 Jan 15;27(2):349-59
Molecular biology of the cell 2016 Jan 15;27(2):349-59
Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo.
Wild F, Khan MM, Straka T, Rudolf R
Autophagy 2016 Dec;12(12):2300-2310
Autophagy 2016 Dec;12(12):2300-2310
Endophilin B2 promotes inner mitochondrial membrane degradation by forming heterodimers with Endophilin B1 during mitophagy.
Wang YH, Wang JQ, Wang Q, Wang Y, Guo C, Chen Q, Chai T, Tang TS
Scientific reports 2016 Apr 26;6:25153
Scientific reports 2016 Apr 26;6:25153
The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs.
Takahashi Y, Tsotakos N, Liu Y, Young MM, Serfass J, Tang Z, Abraham T, Wang HG
Oncotarget 2016 Apr 12;7(15):20855-68
Oncotarget 2016 Apr 12;7(15):20855-68
IFNg-induced Irgm1 promotes tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy.
Dong H, Tian L, Li R, Pei C, Fu Y, Dong X, Xia F, Wang C, Li W, Guo X, Gu C, Li B, Liu A, Ren H, Wang C, Xu H
Oncogene 2015 Oct 16;34(42):5363-71
Oncogene 2015 Oct 16;34(42):5363-71
Loss of endophilin-B1 exacerbates Alzheimer's disease pathology.
Wang DB, Kinoshita Y, Kinoshita C, Uo T, Sopher BL, Cudaback E, Keene CD, Bilousova T, Gylys K, Case A, Jayadev S, Wang HG, Garden GA, Morrison RS
Brain : a journal of neurology 2015 Jul;138(Pt 7):2005-19
Brain : a journal of neurology 2015 Jul;138(Pt 7):2005-19
Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons.
Wang DB, Uo T, Kinoshita C, Sopher BL, Lee RJ, Murphy SP, Kinoshita Y, Garden GA, Wang HG, Morrison RS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 12;34(7):2674-83
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 12;34(7):2674-83
Bif-1 suppresses breast cancer cell migration by promoting EGFR endocytic degradation.
Runkle KB, Meyerkord CL, Desai NV, Takahashi Y, Wang HG
Cancer biology & therapy 2012 Aug;13(10):956-66
Cancer biology & therapy 2012 Aug;13(10):956-66
Scratching beneath the surface: new insights into the functional properties of the lateral occipital area and parahippocampal place area.
Cant JS, Goodale MA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Jun 1;31(22):8248-58
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Jun 1;31(22):8248-58
Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy.
Takahashi Y, Meyerkord CL, Hori T, Runkle K, Fox TE, Kester M, Loughran TP, Wang HG
Autophagy 2011 Jan;7(1):61-73
Autophagy 2011 Jan;7(1):61-73
Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy.
Takahashi Y, Meyerkord CL, Hori T, Runkle K, Fox TE, Kester M, Loughran TP, Wang HG
Autophagy 2011 Jan;7(1):61-73
Autophagy 2011 Jan;7(1):61-73
GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.
Yang J, Takahashi Y, Cheng E, Liu J, Terranova PF, Zhao B, Thrasher JB, Wang HG, Li B
Journal of cell science 2010 Mar 15;123(Pt 6):861-70
Journal of cell science 2010 Mar 15;123(Pt 6):861-70
Endophilin B1/Bif-1 stimulates BAX activation independently from its capacity to produce large scale membrane morphological rearrangements.
Etxebarria A, Terrones O, Yamaguchi H, Landajuela A, Landeta O, Antonsson B, Wang HG, Basañez G
The Journal of biological chemistry 2009 Feb 13;284(7):4200-12
The Journal of biological chemistry 2009 Feb 13;284(7):4200-12
SRC directly phosphorylates Bif-1 and prevents its interaction with Bax and the initiation of anoikis.
Yamaguchi H, Woods NT, Dorsey JF, Takahashi Y, Gjertsen NR, Yeatman T, Wu J, Wang HG
The Journal of biological chemistry 2008 Jul 4;283(27):19112-8
The Journal of biological chemistry 2008 Jul 4;283(27):19112-8
Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis.
Takahashi Y, Coppola D, Matsushita N, Cualing HD, Sun M, Sato Y, Liang C, Jung JU, Cheng JQ, Mulé JJ, Pledger WJ, Wang HG
Nature cell biology 2007 Oct;9(10):1142-51
Nature cell biology 2007 Oct;9(10):1142-51
Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG
Molecular and cellular biology 2005 Nov;25(21):9369-82
Molecular and cellular biology 2005 Nov;25(21):9369-82
Endophilin B1 is required for the maintenance of mitochondrial morphology.
Karbowski M, Jeong SY, Youle RJ
The Journal of cell biology 2004 Sep 27;166(7):1027-39
The Journal of cell biology 2004 Sep 27;166(7):1027-39
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Cuddeback SM, Yamaguchi H, Komatsu K, Miyashita T, Yamada M, Wu C, Singh S, Wang HG
The Journal of biological chemistry 2001 Jun 8;276(23):20559-65
The Journal of biological chemistry 2001 Jun 8;276(23):20559-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone 30A882.1.1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Endophilin B1 was observed. Staining was performed by Histowiz.
