66612-1-IG

antibody from Invitrogen Antibodies

Targeting: MTHFR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66612-1-IG

Provider

Invitrogen Antibodies

Product name

MTHFR Monoclonal Antibody (1F7A3)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: QELLASGLV PGLHFYTLNR EMATTEVLKR LGMWTEDPRR PLPWALSAHP KRREEDVRPI FWASRPKSYI YRTQEWDEFP NGRWGNSSSP AFGELKDYYL FYLKSKSPKE ELLKMWGEEL TSEASVFEVF VLYLSGEPNR NGHKVTCLPW NDEPLAAETS LLKEELLRVN RQGILTINSQ PNINGKPSSD PIVGWGPSGG YVFQKAYLEF FTSRETAEAL LQVLKKYELR VNYHLVNVKG ENITNAPELQ PNAVTWGIFP GREIIQPTVV DPVSFMFWKD EAFALWIEQW GKLYEEESPS RTIIQYIHDN YFLVNLVDND FPLDNCLWQV VEDTLELLNR PTQNARETEA P (307-656 aa encoded by BC053509)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F7A3

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66612-1-IG (MTHFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 66612-1-IG (MTHFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 66612-1-IG (MTHFR antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen tissue slide using 66612-1-IG (MTHFR antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using (Product # 66612-1-IG) (MTHFR antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).