A303-623A

antibody from Invitrogen Antibodies

Targeting: MTHFR

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-623A

Provider

Invitrogen Antibodies

Product name

MTHFR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MTHFR by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MTHFR (Cat. No. A303-623A lot 2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MTHFR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTHFR antibody A303-623A (lot A303-623A-2) used for IP at 3 µg per reaction. MTHFR was also immunoprecipitated by rabbit anti-MTHFR antibody BL12893. For blotting immunoprecipitated MTHFR, A303-623A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.