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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-109293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Human skin tissue lysates, A549, SH-SY-5Y, mouse liver tissue, MCF-7.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APR3 in human skin tissue lysates. Samples were incubated with APR3 polyclonal antibody (Product # PA5-109293), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of APR3 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with APR3 polyclonal antibody (Product # PA5-109293), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of APR3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with APR3 polyclonal antibody (Product # PA5-109293), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of APR3 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with APR3 polyclonal antibody (Product # PA5-109293), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APR3 in paraffin-embedded mouse liver tissue. Samples were incubated with APR3 polyclonal antibody (Product # PA5-109293), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of APR3 in MCF-7 cells (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Samples were incubated with APR3 polyclonal antibody (Product # PA5-109293) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
