Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-27694 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GluR1 Monoclonal Antibody (S355-1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S355-1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references A novel bio-inspired strategy to prevent amyloidogenesis and synaptic damage in Alzheimer's disease.
Catania M, Colombo L, Sorrentino S, Cagnotto A, Lucchetti J, Barbagallo MC, Vannetiello I, Vecchi ER, Favagrossa M, Costanza M, Giaccone G, Salmona M, Tagliavini F, Di Fede G
Molecular psychiatry 2022 Dec;27(12):5227-5234
Molecular psychiatry 2022 Dec;27(12):5227-5234
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GluR1 in rat brain membrane with 10 µg of sample. The sample was incubated with GluR1 monoclonal antibody (Product # MA5-27694) using a dilution of 1:2000 (1 hour at RT), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GluR1 Monoclonal Antibody (S355-1) (Product # MA5-27694) and a 100 kDa band corresponding to GluR1 was observed in brain tissues. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Liver (Lane 2), Rat Brain (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GluR1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GluR1 monoclonal antibody (Product # MA5-27694) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GluA1/GluR1 Antibody, D) Merged image. Magnification: 60x.
