17682-1-AP

antibody from Invitrogen Antibodies

Targeting: AHNAK2 C14orf78

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17682-1-AP

Provider

Invitrogen Antibodies

Product name

AHNAK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EQPVDLNLP LEAPPISKVR VHIQGAQVES QEVTIHSIVT PEFVDLSVPR TFSTQIVRES EIPTSEIQTP SYGFSLLKVK IPEPHTQARV YTTMTQHSRT QEGTEEAPIQ ATPGVDSISG DLQPDTGEPF EMISSSVNVL GQQTLTFEVP SGHQLADSCS DEEPAEILEF PPDDSQEATT PLADEGRAPK DKPESKKSGL LWFWLPNIGF SSSVDETGVD SKNDVQRSAP IQTQPEARPE AELPKKQEKA GWFRFPKLGF SSSPTKKSKS TEDGAELEEQ KLQEETITFF DARESFSPEE KEEGELIGPV GTGLDSRVMV TSAARTELIL PEQDRKADDE SKGSGLGPNE G (134-483 aa encoded by BC049216)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17682-1-AP ( AHNAK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using (Product # 17682-1-AP) (AHNAK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).