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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 49-1037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAZ2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors.
Wiechens N, Singh V, Gkikopoulos T, Schofield P, Rocha S, Owen-Hughes T
PLoS genetics 2016 Mar;12(3):e1005940
PLoS genetics 2016 Mar;12(3):e1005940
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using the anti-TIP5 crude serum. Nuclear extracts (150 µg) from NIH3T3 or HeLa cells were analyzed by western blot using the anti-TIP5 crude serum (Product # 49-1037), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween-20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assays were performed using NIH3T3 and HeLa cells, the anti-TIP5 crude serum (Product # 49-1037), and optimized PCR primer sets for qPCR (for the promoter and the coding region of the 28s ribosomal RNA gene). Each ChIP assay used 100 µg sheared chromatin (average fragment lengths between 200 and 400 bp, incubated overnight at 4°C) and either 5 µl crude serum against TIP5 or 5 µl IgG which was used as negative IP control. This figure shows the recovery by the TIP5 crude serum and by IgG (set to 1), normalized to the input DNA. These results show that, in both HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28s rRNA gene.
