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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 16617-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: VAEPELMGT PDGTCYPPPP VPRQASPQNL GTPGLLHPQT SPLLHSLVGQ HILSVQQFTK DQMSHLFNVA HTLRMMVQKE RSLDILKGKV MASMFYEVST RTSSSFAAAM ARLGGAVLSF SEATSSVQKG ESLADSVQTM SCYADVVVLR HPQPGAVELA AKHCRRPVIN AGDGVGEHPT QALLDIFTIR EELGTVNGMT ITMVGDLKHG RTVHSLACLL TQYRVSLRYV APPSLRMPPT VRAFVASRGT KQEEFESIEE ALPDTDVLYM TRIQKERFGS TQEYEACFGQ FILTPHIMTR AKKKMVVMHP MPRVNEISVE VDSDPRAAYF RQAENGMYIR MALLATVLGR F (502-851 aa encoded by BC014178)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP ( CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug.
