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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A301-374A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse CAD by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody (Product # A301-374A; lot A301-374A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody A301-374A (lot A301-374A-2) used for IP at 6 µg per reaction. CAD was also immunoprecipitated by rabbit anti-CAD antibody A302-372A. For blotting immunoprecipitated CAD, A301-374A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse CAD by western blot. Samples: Whole cell lysate from He L a (5, 15 and 50 µg), 293T (50 µg), and mouse NIH3T3 (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody A301-374A (lot A301-374A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CAD by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAD (Product # A301-374A; lot 3) used at a dilution of 1:1,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CAD by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CAD (Cat. No. A301-374A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody (Product # A301-374A; lot A301-374A-3) used for IP at 6 µg per reaction. CAD was also immunoprecipitated by a previous lot of this antibody (lot A301-374A-2) and rabbit anti-CAD antibody (Product # A301-372A). For blotting immunoprecipitated CAD (Product # A301-374A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
