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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- MAB4230 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat DARPP-32 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
- Reactivity
- Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UD71.2- Isotype
- IgG
- Antibody clone number
- 375604
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease.
Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease.
Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity.
Early life exposure to a high fat diet promotes long-term changes in dietary preferences and central reward signaling.
Schmidt ME, Buren C, Mackay JP, Cheung D, Dal Cengio L, Raymond LA, Hayden MR
BMC biology 2018 Jun 27;16(1):58
BMC biology 2018 Jun 27;16(1):58
Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease.
Gomez-Pastor R, Burchfiel ET, Neef DW, Jaeger AM, Cabiscol E, McKinstry SU, Doss A, Aballay A, Lo DC, Akimov SS, Ross CA, Eroglu C, Thiele DJ
Nature communications 2017 Feb 13;8:14405
Nature communications 2017 Feb 13;8:14405
Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity.
Chen M, Wan Y, Ade K, Ting J, Feng G, Calakos N
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Jun 29;31(26):9563-73
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Jun 29;31(26):9563-73
Early life exposure to a high fat diet promotes long-term changes in dietary preferences and central reward signaling.
Teegarden SL, Scott AN, Bale TL
Neuroscience 2009 Sep 15;162(4):924-32
Neuroscience 2009 Sep 15;162(4):924-32
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse/Rat DARPP-32 by Western Blot. Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
