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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-32113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DARPP-32 Recombinant Rabbit Monoclonal Antibody (SU0329)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SU0329
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DARPP-32 in mouse brain lysate using a DARPP-32 Monoclonal antibody (Product # MA5-32113) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of DARPP-32 in SH-SY-5Y cells using a DARPP-32 Monoclonal antibody (Product # MA5-32113) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of DARPP-32 in MCF-7 cells using a DARPP-32 Monoclonal antibody (Product # MA5-32113) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DARPP-32 of paraffin-embedded rat brain tissue using a DARPP-32 Monoclonal antibody (Product #MA5-32113). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DARPP-32 of paraffin-embedded Human breast carcinoma tissue using a DARPP-32 Monoclonal antibody (Product #MA5-32113). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DARPP-32 of paraffin-embedded Human gastric carcinoma tissue using a DARPP-32 Monoclonal antibody (Product #MA5-32113). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of DARPP-32 in Hela cells using a DARPP-32 Monoclonal Antibody (Product # MA5-32113) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
