Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22382 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOMER3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1. Predicted reactivity: Mouse (85%), Rat (86%), Rhesus Monkey (98%). IHC (P): suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HOMER3 Polyclonal Antibody (Product # PA5-22382). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 10% SDS PAGE. HOMER3 Polyclonal Antibody (Product # PA5-22382) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Homer-3 in methanol-fixed HeLa cells using a Homer-3 polyclonal antibody (Product # PA5-22382) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HOMER3 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: HOMER3 Polyclonal Antibody (Product # PA5-22382) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HOMER3 Polyclonal Antibody detects Homer3 protein at cytoplasm on human Papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Papillary adenocarcinoma. HOMER3 Polyclonal Antibody (Product # PA5-22382) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
