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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- PA5-87716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GOLGA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MFKKLKQKIS EEQQQLQQAL APAQASSNSS TPTRMRSRTS SFTEQLDEGT PNRESGDTQS FAQKLQLRVP SVESLFRSPI KESLFRSSSK ESLVRTSSRE SLNRLDLDSS TASFDPPSDM DSEAEDLVGN SDSLNKEQLI QRLRRMERSL; Positive Samples: U-87MG, HeLa, SW480, A-549, MCF7, Mouse heart, Mouse brain; Cellular Location: Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.02 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GOLGA4 in extracts of various cell lines using GOLGA4 Polyclonal Antibody (Product # PA5-87716) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GOLGA4 was performed in C6 cells using GOLGA4 Polyclonal Antibody (Product # PA5-87716) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GOLGA4 was performed in HeLa cells using GOLGA4 Polyclonal Antibody (Product # PA5-87716) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GOLGA4 was performed in L929 cells using GOLGA4 Polyclonal Antibody (Product # PA5-87716) at a dilution of 1:100. Blue: DAPI for nuclear staining.
